The TranScreen LE System as an alternative to Fluorography

Fluorography, is method used to increase the sensitivity of medium and low energy beta isotopes embedded in gel slices. This methodology requires the use of fluorochromes, commercially available. These fluorochromes have varying levels of toxicity depending on the fluorochrome used. Samples subjected to flourography must be disposed of as mixed waste. Another disadvantage to fluorography is the method is a non-passive; meaning that once a sample has been treated with the fluorochrome the sample cannot be used in any other experiments, and must be disposed of as a mixed waste.

The TranScreen LE improves resolution and detection sensitivities of isotopes in gels (i.e. 35 S and 3 H). 3 H samples in gels must be blotted onto a membrane, since 3 H particles have insufficient energy to escape a dried gel. The blot is then simply applied to the TranScreen LE system (see general purpose usage). For 35 S & 33 P we also recommend blotting the gel to optimize sensitivity, however this is not absolutely necessary as 35 S and 33 P particles have sufficient energy to escape a thin dried gel. Another advantage of the TranScreen system over fluorography (besides improved sensitivity and resolution) is that the sample is not contaminated by a fluoro and therefore can easily be reused in additional experiments. Blotted samples offer greater sensitivity and possibly more reliability than using flourography with a gel (Quemeneur and Simonnet,1995, BioTechniques 18, 100-103).

Common Questions about TranScreen LE as an alternative to Fluorography: 

Question: What membrane should I use to blot my sample?
The choice of membrane is dictated by the sample itself. i.e. a nylon membrane is recommended for nucleic acid, and PVDF is recommended for protein samples.

Question: Is it necessary to use a blocking agent?
The use of a blocking agent is not necessary for the performance of the BioMax TranScreen system. If further experiments (i.e. Southern or Western) are planned for the blot after exposure to the TranScreen, then the subsequent experiment (s) will dictate whether or not it is necessary to use a blocking agent.