TranScreen LE Sensitivity Film Comparisons

35S Slot-Blot Images Captured Using...

Transcreen LE 35S

 
Figure 1 
Comparative analysis of BioMax TranScreen LE/BioMax MS film to commonly used autoradiography systems for 35S. The experimental design comprises a cellular protein labeled with 35S-methionine, slot-blotted in a band format onto a membrane.

 

BioMax Transcreen/BioMax MS image directly compared to 3H standard file.

Transcreen LE 3H

 
Figure 2 
Comparative analysis of BioMax TranScreen LE/BioMax MS film to commonly used autoradiography system for 3H on standard slides.

Comparative images of 35S and 3H demonstrates the enhanced detection sensitivity of the BioMax TranScreen LE/BioMax MS film system (figures 1 & 2). Note, that the TranScreen LE system image is similar to a band of similar gray level captured by film alone; maintaining a sharp image implies that the TranScreen LE system conserves much of the spatial resolution inherent to the sample.

 

Response of Screen/Film Systems to 32P

Transcreen LE Comparative Analysis

 
Figure 3 
Comparative analysis of BioMax TranScreen LE/BioMax MS film to generic screen/X-OMAT AR film system for 32P.

Figure 3 demonstrates the enhanced spatial resolution and sensitivity of the BioMax TranScreen LE/BioMax MS film compared to generic intensifying screen/X-Omat AR film combination for 32P. Note: for 4-5 X more sensitivity for penetrating radiation such as 32P and 125I refer to BioMax TranScreen HE.